Which enzyme pair are isocaudomers?
Isocaudomers are distinct restriction endonucleases that recognize different nucleotide sequences yet generate identical cohesive ends, permitting intermolecular ligation. XhoI recognizing 5'-C^TCGAG-3' and SalI recognizing 5'-G^TCGAC-3' represent classic example; both release fragments with 5' overhang TCGA. When such fragments are mixed, complementary ends anneal and are joined by ligase, forming CTCGAC or GTCGAG chimeras refractory to cleavage by either enzyme. Isocaudomer compatibility expands cloning flexibility, allowing insertion of a SalI fragment into XhoI-linearized vector when conve
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
