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#haploid plants

3 public questions tagged with this topic.

Plant tissue culture 2

Plants produced via androgenesis are initially:

Plants produced via androgenesis are initially haploid bearing single chromosome set n derived from meiotic reduction of diploid mother cells into microspores. After meiosis each microspore contains recombined haploid genome; when cultured without fertilization embryogenic divisions maintain this n number, so tissues leaves roots show halved DNA content compared to diploid control confirmed by flow cytometry and chromosome counting revealing for example 7 chromosomes in barley haploid versus 14 diploid. Such haploids exhibit reduced vigor smaller organs small stomatal guard cells and complete

Ref: Kasha & Kao 1970 haploid plants; Maluszynski et al., FAO doubled haploid manual.

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Plant tissue culture 2

Androgenesis is commonly achieved by culture of:

Androgenesis is commonly accomplished through culture of anthers or isolated microspores pollen at uninucleate stage, landmark discovery by Guha and Maheshwari 1964 in Datura innoxia demonstrating that anthers could produce haploid embryos on MS medium. Most responsive microspores are at mid uninucleate to early binucleate stage where gametophytic commitment is still reversible; vacuolate microspores have large central vacuole and peripheral nucleus poised for embryogenic switch. Anthers excised from surface sterilized buds given cold pretreatment 4 degrees for several days or heat shock 33 de

Ref: Nitsch & Nitsch 1969 androgenesis; Germaná 2011 anther culture history; NCBI NBK21429.

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Plant tissue culture 2

Androgenesis results in plants genetically identical to:

Androgenesis results in plants whose nuclear genetic constitution is identical to male parent gamete contribution because development originates from male gametophyte microspore. Microspores formed after meiosis in anther tapetum carry recombined haploid genome representing segregation products of diploid male parent heterozygous loci. Under stress pretreatment cold starvation heat and culture conditions gametophytic program that would normally produce mature binucleate pollen with vegetative generative cells is aborted, sporophytic pathway triggered leading to repeated mitotic divisions formi

Ref: Guha & Maheshwari 1964 androgenesis; Touraev et al., Trends Plant Sci microspore embryogenesis.

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