Selective agent commonly used in hairy root culture is:
Effective recovery of transformed roots requires stringent chemical selection distinguishing transgenic from non-transformed background. Hygromycin B, aminoglycoside antibiotic, inhibits protein biosynthesis by stabilizing tRNA-ribosome interaction causing misreading. Plant cells are highly sensitive, with lethal concentration around 20 mg per liter for many dicots. Transformation vectors carry hpt gene under NOS promoter encoding hygromycin phosphotransferase that phosphorylates antibiotic hydroxyl group, preventing ribosome binding. Co-transformation with Ri T-DNA and binary vector results i
Ref: Beck Gene 1982 hpt hygromycin; Waldron Plant Mol Biol 1985; NCBI NBK131103 markers; Jefferson Plant Cell Rep 1987 hygromycin hairy root selection.
