Which histone chaperone deposits H3-H4 during DNA replication?
During S phase newly synthesized histones deposited behind replication fork by dedicated chaperone complexes ensuring rapid nucleosome reassembly and genome stability. CAF-1 complex comprising three subunits p150, p60, p48 specifically binds proliferating cell nuclear antigen PCNA at replication fork and hands H3-H4 dimers to assembling DNA, coupling nucleosome formation to synthesis. NAP1 chaperones H2A-H2B dimers, ASF1 transfers H3-H4 dimers to CAF-1 and HIRA variant pathway, FACT facilitates transcription elongation through nucleosomes allowing polymerase passage. CAF-1 depletion causes S p
Ref: Smith and Stillman 1989 CAF-1 Discovery; Alberts et al., Chapter 5: CAF-1 Deposits H3-H4 During Replication
