Glyphosate inhibits which enzyme?
Glyphosate N-phosphonomethyl glycine inhibits key enzyme of aromatic amino acid pathway. 5-enolpyruvylshikimate-3-phosphate synthase normally condenses shikimate-3-phosphate and phosphoenolpyruvate to EPSP in chloroplast stroma, penultimate step of shikimate pathway. Glyphosate mimics PEP, occupying PEP binding site in EPSPS-S3P complex forming stable dead-end complex preventing enolpyruvyl transfer. Plants cannot synthesize chorismate, precursor of phenylalanine, tyrosine, tryptophan, causing accumulation of shikimate, depletion of aromatic amino acids, cessation of protein synthesis, and dea
Ref: Steinrücken Biochem Biophys Res Commun 1980 glyphosate EPSPS; Funke PNAS 2006 Roundup Ready molecular basis; NCBI NBK22387; https://www.pnas.org/doi/10.1073/pnas.0603638103