Wide hybridization followed by genome elimination is used in DH production of:
Wide hybridization with chromosome elimination exploits post-zygotic incompatibility where chromosomes of one parent are selectively lost during early embryonic divisions due to centromere incompatibility. In cereal DH production, wheat Triticum aestivum crossed as female to maize Zea mays as pollinator leads to fertilization but maize chromosomes fail to attach to wheat spindle due to centromere divergence and asynchronous mitosis, becoming micronuclei and eliminated within few divisions. Resulting embryo contains only haploid wheat chromosomes, rescued via embryo culture on artificial medium
Ref: Laurie DA & Bennett MD 1988 – Wheat × maize DH; Kasha KJ & Kao KN – Bulbosum method mechanism
