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#gene silencing

25 public questions tagged with this topic.

ZOOLOGY

Which technique uses RNA interference to silence specific gene expression in pests?

RNA interference (RNAi) is used to silence specific gene expression, including in pests for crop protection. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.

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Transgenic Plant -2

Flavr Savr tomato was developed by silencing which gene?

Flavr Savr tomato pioneered commercial transgenic fruit improvement by addressing postharvest softening. Ripening in tomato involves ethylene induced expression of polygalacturonase encoded by pg gene, endo-acting enzyme depolymerizing homogalacturonan in middle lamella leading to cell separation and fruit softening. Over-softening limits shelf life and transportability. Calgene developed antisense construct placing pg cDNA in reverse orientation behind CaMV 35S promoter, transformed into tomato cultivar via Agrobacterium. Antisense transcript forms duplex with endogenous pg mRNA, reducing tra

Ref: Sheehy PNAS 1988 antisense polygalacturonase Flavr Savr; Kramer CRC Crit Rev 1994 history; NCBI NBK131103; PubMed 16593109 tomato antisense.

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Gene Therapy

Targeted inhibition of gene expression can be achieved by:

Antisense oligonucleotides synthetic single-stranded nucleic acids 16-20 nucleotides bearing phosphorothioate backbone replacing nonbridging oxygen sulfur increasing nuclease resistance albumin binding prolonging half-life, 2 prime O-methoxyethyl sugar increasing affinity Tm 2 degrees per modification reducing immune stimulation TLR7/8, locked nucleic acids constraining ribose C3 endo enhancing hybridization strength. Binding target mRNA via Watson-Crick pairing forming heteroduplex nucleus or cytoplasm, two mechanisms dominate: recruitment endogenous RNase H1 hydrolyzing RNA strand DNA-RNA hy

Ref: FDA Antisense Oligonucleotide Guidance 2023; Nature Reviews Drug Discovery Antisense Mechanism 2021; Alberts Molecular Biology RNA Targeting Chap 8.

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