Orientation of a cloned passenger gene cassette in a binary plasmid vector, containing the expression cassette of a sele
In biotechnology, genetic engineering dictates that vector design and pathway extension enable vitamin production. Therefore a combination of passenger gene-specific and vector-specific primers is correct as it utilizes recombinant DNA and metabolic engineering, supported by mechanistic studies and conserved across related systems.
Ref: Primrose and Twyman, Principles of Gene Manipulation and Genomics, 8th Edition, Chapter 2, Recombinant DNA Technology, discusses Golden Rice development, exogenous genes, metabolic engineering and vitamin A pathway extension supporting applications.
