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#expression restriction

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Several experiments have shown leaky/deregulated expression of reporter genes viz., GUS from plant promoters in Agrobact

Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates Use of intron(s) within the transgene sequence because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.

Ref: Agrawal, Quantitative Aptitude for Competitive Examinations, Chapter 5, Logical Reasoning and Probability, covers ratios, percentages, series completion, time-distance and analytical reasoning explaining problem-solving strategies for competitive assessments.