Which of the following is the best method to determine the phospholipid asymmetry in a plasma membrane?
In biochemistry, enzyme catalysis and lipid structure dictates that substrate affinity, transition state stabilization and phospholipid composition govern function. Therefore Thin layer chromatography is correct as it reflects catalytic efficiency and membrane organization, supported by mechanistic studies and conserved across related systems.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 7th Edition, Chapter 6, Enzyme Kinetics and Regulation, discusses Michaelis-Menten equation, transition state stabilization and allosteric control supporting enzyme function.
