Skip to content

#eukaryotes

34 public questions tagged with this topic.

The actin cytoskeleton in prokaryotic cells is functionally similar to which of the following eukaryotic proteins?

Prokaryotic cells maintain rod shape and organize cell wall synthesis without eukaryotic compartments using cytoskeletal homologs sharing ancient ATPase fold. MreB, belonging to actin Hsp70 superfamily, shares structural core of five conserved sequence motifs forming ATP binding pocket between domains IA and IIA. Purified MreB polymerizes into ATP dependent antiparallel double filaments that rotate circumferentially beneath cytoplasmic membrane driven by cell wall elongation machinery. It scaffolds elongasome complex containing RodA glycosyltransferase, PBP2 transpeptidase and MreC MreD, directing insertion of new peptidoglycan hoops to maintain lateral wall. Depletion or inhibition by small molecule A22 causes rod to sphere transition confirming morphogenetic role, similar to actin controlling shape in eukaryotes. Keratin and vimentin are metazoan intermediate filament proteins absent in most bacteria and archaea, while ActA is Listeria monocytogenes surface protein that activates host Arp2/3 complex to form actin comet tails for motility, not bacterial cytoskeleton. Thus prokaryotic actin analog best matching functional criteria is MreB protein.

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 16: Cytoskeleton and Bacterial Homologs MreB.

The bacterial reproduction process of binary fission differs from mitosis in that:

Binary fission represents prokaryotic division program fundamentally distinct from eukaryotic mitosis in mechanism and regulation. Initiation begins with replication of single circular chromosome from unique origin oriC proceeding bidirectionally via replisomes containing DNA polymerase III. Segregation of origins is mediated by ParABS partitioning system and SMC condensin complexes moving DNA toward opposite poles as cell elongates via MreB-guided peptidoglycan insertion. Division site selection uses precise regulatory systems: oscillating MinCDE proteins concentrate MinC inhibitor at poles preventing polar Z-ring assembly, and nucleoid occlusion proteins SlmA in E. coli and Noc in B. subtilis bind chromosomal DNA blocking FtsZ polymerization over nucleoids, ensuring tubulin homolog FtsZ assembles into contractile Z-ring only at mid-cell between separated chromosomes. FtsZ then recruits FtsA, ZipA, FtsQ, FtsL, FtsW and FtsI transpeptidase that synthesize septal wall and drive constriction via GTP hydrolysis. No microtubule spindle, kinetochores, centrosomes or chromosome condensation occurs, and daughters are monoploid identical cells, making absence of spindle the defining difference from mitosis.

Ref: Madigan et al., Brock Biology of Microorganisms, 16th ed., Chapter 4: Binary Fission and Absence of Spindle Apparatus.

Two cells show the following features: Cell X is relatively small, multiplies rapidly, contains naked genetic material a

Small size, rapid multiplication, naked DNA and absence of membrane-bound organelles identify X as prokaryotic. An organised nucleus and extensive compartmentalisation identify Y as eukaryotic. Eukaryotes include protists, plants, animals and fungi.

Ref: NCERT Class 11 Biology Chapter 8: Cell: The Unit of Life Prokaryotic Cell - Structure and Components