A closed circular plasmid of length 5000 base pairs is completely relaxed in aqueous buffer. If the plasmid is put in 80
The principle of nucleic acid structure and recombination explains that hairpin formation, Holliday junction resolution and translation control ensure fidelity. Hence It will become positively supercoiled fits best because it matches molecular mechanisms, as documented in authoritative sources on molecular biology.
Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, Chapter 9, Transcription and RNA Processing, describes RNA hairpin formation, secondary structures and regulatory mechanisms controlling gene expression.
