#EDTA

Animal Tissue Culture 2

EDTA enhances the activity of:

Ethylenediaminetetraacetic acid is polyaminocarboxylic acid with hexadentate chelation property exhibiting high affinity for divalent cations calcium and magnesium. Many cell adhesion molecules are calcium-dependent: classical cadherins require calcium to rigidify extracellular repeats for homophilic trans-interaction, and integrins need divalent ions for conformational activation and ligand binding to RGD motifs in extracellular matrix. By sequestering calcium, EDTA disrupts these homophilic bonds, loosens adherens junctions, desmosomes, and focal adhesions, enhancing penetration of trypsin t

Ref: Alberts MBoC 6th Ed Ch.19 Cadherin Ca2+ dependent adhesion; Freshney EDTA chelates Ca2+ enhances trypsin activity for cell detachment.

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Animal Tissue Culture 1

EDTA acts as an inhibitor of:

Metalloprotease class distinguished by requirement zinc ion for catalytic mechanism and often calcium for structural integrity. Active site contains conserved motif HEXXH where histidines coordinate zinc plus third ligand histidine or glutamate forming tetrahedral geometry polarizing water molecule held nearby as hydroxide ion via general base glutamate abstracting proton making hydroxide nucleophile that attacks peptide carbonyl carbon forming tetrahedral transition state stabilized by zinc positive charge and nearby tyrosine oxyanion hole. Removal of zinc abolishes activity irreversibly. Eth

Ref: Lipscomb & Strater Chem Rev 1996 metalloprotease zinc HEXXH; Sigma EDTA chelation Zn Ca MMP inhibition detachment synergy.

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DNA & RNA isolation

What is the role of EDTA in DNA isolation?

EDTA (ethylenediaminetetraacetic acid) is a chelating agent essential in DNA isolation buffers. It binds divalent cations like Mg2+ and Ca2+ with high affinity, forming stable coordination complexes. Mg2+ is a crucial cofactor for DNases that degrade DNA and for nucleases that destabilize membranes. By sequestering Mg2+, EDTA irreversibly inhibits endogenous nuclease activity, protects genomic DNA from enzymatic degradation, and helps destabilize cell walls by removing membrane-stabilizing cations. It does not denature proteins directly, bind nucleic acids specifically, or degrade RNA; its fun

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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Cell Communication and Cell Adhesion

Removal of Ca2+ by EDTA leads to loss of adhesion mediated by

Cadherins, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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MINERAL Transport+PYQS

EDTA and DTPA are examples of:

Chelators (C) is correct here. This is core MINERAL Transport: once you know the definition or pathway step, Chelators is the clear fit. The wrong ones are A) Enzymes; B) Phytohormones; D) Transport proteins. If the topic is about gradients or potentials, water/solutes move from higher to lower of the relevant quantity.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.

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