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#dynamin

3 public questions tagged with this topic.

Protein Sorting and Trafficking

Which protein is required for vesicle scission during receptor-mediated endocytosis?

Final separation stage clathrin-coated pit formation yielding free vesicle requires enzymatic fission beyond coat polymerization. Clathrin assembly with adaptor AP2 and BAR proteins epsin, amphiphysin, endophilin generates deeply invaginated bud connected plasma membrane narrow tubule neck diameter around 20 nm bilayer. Separation demands mechanical constriction cutting neck. Specialist protein dynamin large 96 kDa GTPase family founding member N-terminal GTPase domain, middle stalk, PH domain binding PI(4,5)P2 enriched site, GED and PRD binding SH3, polymerizes helical collar around neck. Rec

Ref: Kaksonen & Roux, Nat Rev Mol Cell Biol: Dynamin scission in clathrin-mediated endocytosis.

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Protein Sorting and Trafficking

The process of vesicle scission during clathrin-mediated endocytosis requires:

Formation of endocytic vesicle demands energetically unfavorable fission of neck connecting bud to parent membrane against line tension and bending resistance. In clathrin-mediated pathway adaptor AP2 and accessory proteins FCHo, epsin together with clathrin lattice driving polymerization stabilize deeply invaginated bud with omega shape. Constriction of neck performed by dynamin large 96 kDa GTPase assembling into helical oligomer about two turns around neck 50 nm diameter PH domain binding PI(4,5)P2. Polymerization aligns G domains enhancing GTPase 100 fold. Upon concerted GTP binding and co

Ref: Schmid & Frolov, Annu Rev Cell Dev Biol: Dynamin GTP hydrolysis drives membrane scission.

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Protein Sorting and Trafficking

What is the role of dynamin in clathrin-coated vesicle formation?

Clathrin-coated vesicle formation ends with detachment step demanding membrane remodeling fission against line tension. While clathrin lattice with adaptor AP2 and accessory proteins like epsin concentrate cargo and deform membrane into omega pit, polymerization does not cut bilayer. Instead large GTPase dynamin recruited via SH3 interactions with amphiphysin, endophilin, intersectin binding proline-rich domain. Dynamin 100 kDa possesses G domain, PH domain binding PI(4,5)P2, stalk for dimerization and GED. It assembles into helical polymer around vesicle neck about 13 dimers per turn. Upon GT

Ref: Alberts et al., MBC Chapter 13: Dynamin GTPase mediates vesicle scission in endocytosis.

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