MDCK cells are widely used to study:
MDCK cells are premier model for epithelial physiology because they form highly polarized monolayers with distinct apical membrane enriched in glycoproteins with GPI anchors and basolateral membrane containing receptors for growth factors, enabling study of protein sorting signals such as tyrosine motifs and raft-dependent trafficking via Par3-Par6-aPKC polarity complex. They develop tight junctions that restrict paracellular diffusion, allowing measurement of drug permeability, P-glycoprotein mediated efflux, and toxicant effects on barrier integrity. In influenza research, MDCK is gold stand
Ref: Simons K & Fuller SD Annu Rev Cell Biol 1985 MDCK polarity; WHO Manual Influenza diagnostics: MDCK culture influenza virus study.
