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#doubled haploids

3 public questions tagged with this topic.

Plant Breeding 4

Main advantage of DH in breeding is:

Primary bottleneck in conventional line breeding is time required to attain homozygosity through repeated selfing, typically six generations to reach acceptable uniformity and distinctness for release. Doubled haploid technology converts heterozygous material into completely homozygous lines immediately, compressing 5-6 year inbreeding cycle into less than 18 months including haploid induction and doubling steps. Haploid inducer lines or anther culture produce haploid embryos, chromosome doubling yields instant true-breeding lines that can be multiplied for yield trials in next season with hig

Ref: Geiger HH & Gordillo GA. 2009 Plant Breeding Reviews – DH rapid homozygosity; Weyen 2008 J

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Plant Breeding 4

Wide hybridization followed by genome elimination is used in DH production of:

Wide hybridization with chromosome elimination exploits post-zygotic incompatibility where chromosomes of one parent are selectively lost during early embryonic divisions due to centromere incompatibility. In cereal DH production, wheat Triticum aestivum crossed as female to maize Zea mays as pollinator leads to fertilization but maize chromosomes fail to attach to wheat spindle due to centromere divergence and asynchronous mitosis, becoming micronuclei and eliminated within few divisions. Resulting embryo contains only haploid wheat chromosomes, rescued via embryo culture on artificial medium

Ref: Laurie DA & Bennett MD 1988 – Wheat × maize DH; Kasha KJ & Kao KN – Bulbosum method mechanism

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Plant Breeding 4

Doubled haploids achieve homozygosity in:

Conventional pedigree inbreeding reduces heterozygosity by half each selfing generation, requiring F6-F7 to attain >98% homozygosity, a process taking 5-6 years. Doubled haploid approach collapses this timeline to single step through manipulation of gametic pathway. Haploid plant carrying n chromosomes is induced via anther culture, isolated microspore culture, ovule culture, or wide hybridization-induced chromosome elimination such as wheat × maize system. Haploid set containing single allele per locus is then doubled chemically with colchicine or spontaneously through endomitosis, producing

Ref: Snape JW. Doubled haploid breeding; Dwivedi et al. 2015 – One-generation homozygosity review

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