The Nieuwkoop center is located at the:
Nieuwkoop center comprises dorsalmost vegetal blastomeres directly beneath future organizer, characterized by highest nuclear beta-catenin accumulation after cortical rotation opposite sperm entry, expressing Siamois, Twin and high Nodal-related genes. Positioned at vegetal edge of gray crescent, these endodermal cells never involute but induce overlying dorsal marginal zone to become Spemann organizer via vertical Nodal signaling. Ventral vegetal cells lacking beta-catenin and Siamois induce ventral mesoderm via BMPs. Animal pole and blastocoel lack this inductive capacity, confirming dorsovegetal location essential for dorsal axis specification.
Ref: Gilbert, Developmental Biology, 12th ed., Chapter 9: Nieuwkoop center location at dorsalmost vegetal cells.