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#DNA uptake

7 public questions tagged with this topic.

Molecular Biology and Cell Biology

Which one of the following agents stimulates direct DNA uptake by protoplasts?

Mechanistically, nucleic acid structure and recombination involves hairpin formation, Holliday junction resolution and translation control ensure fidelity. This validates Polyethylene glycol because it matches molecular mechanisms, a pattern repeatedly demonstrated in molecular biology research.

Ref: Lodish et al., Molecular Cell Biology, 8th Edition, Chapter 8, RNA Processing and Translation, covers mRNA hairpins, puromycin mechanism and aminoacyl-tRNA function governing protein synthesis.

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Cell Biology

Direct DNA uptake by protoplasts can be stimulated by

This outcome reflects nucleic acid structure and recombination, where hairpin formation, Holliday junction resolution and translation control ensure fidelity. Option A captures this correctly because it matches molecular mechanisms, consistent with textbook descriptions and experimental observations in molecular biology.

Ref: Lodish et al., Molecular Cell Biology, 8th Edition, Chapter 8, RNA Processing and Translation, covers mRNA hairpins, puromycin mechanism and aminoacyl-tRNA function governing protein synthesis.

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Microbial genetics

Transformation involves uptake of DNA that is

Transformation involves competent bacteria binding extracellular naked DNA released from lysed cells and importing single strand through competence pseudopilus and Com proteins. No protein coat or phage capsid surrounds DNA; it is deproteinized, often as linear fragments, protected only by its own chemical stability. Once inside, homologous sequences integrate via RecA-mediated recombination replacing recipient allele. Competence develops in Bacillus subtilis, Streptococcus pneumoniae, Haemophilus. Naked nature is proven by DNase sensitivity: addition of DNase destroys transforming activity, u

Ref: Griffiths et al., Introduction to Genetic Analysis, 12th ed., Chapter 6: Bacterial Transformation Mechanism

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