19 public questions tagged with this topic.
Vectors, such as plasmids, are used to carry and replicate foreign DNA inside host lls for cloning. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.
Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.
Recombinant DNA technology involves combining DNA from different sources to form recombinant DNA for_x000D_ various applications, including gene cloning.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Biosafety and Overview of Applications
A sterile environment is crucial in a bioreactor to prevent contamination and ensure the growth of only the desired recombinant bacteria.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Bioreactors and Downstream Processing
Recombinant DNA technology enables the insertion of specific genes into host organisms for desired traits.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Bioreactors and Downstream Processing
Fermentation of alcohol is a natural microbial process and does not necessarily involve recombinant DNA technology.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Bioreactors and Downstream Processing
The first step in recombinant DNA technology is isolating the desired DNA, which is then cut, inserted into a vector, and introduced into a host.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Polymerase Chain Reaction - PCR and Applications
The first step in recombinant DNA technology is isolating the gene of interest before insertion into a vector.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Gel electrophoresis is used to separate DNA fragments based on size, allowing analysis and purification of DNA samples.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Recombinant DNA technology is widely used to develop genetically modified crops with improved traits.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
SDS-PAGE is used for protein separation, while recombinant DNA technology involves steps such as DNA isolation, ligation, and transformation.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
DNA ligase joins DNA fragments by forming phosphodiester bonds, allowing successful recombination.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Competent Host and Gene Transfer Methods
Restriction enzymes cut DNA at specific recognition sequences, which allows precise genetic modifications.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Principles of Biotechnology - Genetic Engineering
