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Restriction enzymes require specific buffer conditions, including optimal pH and ionic strength, to function properly.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Tools - Restriction Enzymes - Molecular Scissors
Hind II was the first restriction enzyme discovered, which specifically cuts DNA at a particular recognition sequence.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Tools - Restriction Enzymes - Molecular Scissors
Arber, Smith, and Nathans discovered restriction enzymes, which are essential tools in recombinant DNA technology.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Tools - Restriction Enzymes - Molecular Scissors
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