Cooling centrifuge is mainly used in cell culture for:
Refrigerated cooling centrifuges operating at 4°C are essential during preparation of animal cells for cryopreservation and extraction of temperature-sensitive macromolecules. During harvesting for freezing, cells undergo trypsin-EDTA detachment releasing intracellular proteases, nucleases, phosphatases and apoptotic mediators. Low temperature markedly reduces metabolic rate, enzymatic degradation, and membrane lipid fluidity changes that predispose cells to lysis. Cells are pelleted at low g-force 100-300 g to avoid shear stress, washed to remove serum and residual trypsin that would interfer
Ref: Freshney Culture of Animal Cells Ch.22 Cryopreservation; ATCC Animal Cell Culture Guide: cold centrifugation before freezing preserves viability.
