Which technique does NOT study conformational dynamics?
Techniques monitoring conformational dynamics must sense time-dependent structural changes: NMR chemical exchange and relaxation, circular dichroism thermal unfolding, fluorescence resonance energy transfer distance fluctuations, and single-molecule imaging. Mass spectrometry measures mass-to-charge ratio, providing stoichiometry, post-translational modifications, and sequencing via fragmentation, but conventional measurements occur in gas phase after ionization, losing real-time solution dynamics unless combined with hydrogen-deuterium exchange pulse labeling or ion mobility. Thus among optio
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
