ChIP-on-chip allows:
ChIP-on-chip integrates chromatin immunoprecipitation with DNA microarray technology for high-throughput genome-wide mapping of protein-DNA interactions. After formaldehyde crosslinking, chromatin shearing and immunoprecipitation using antibody against transcription factor or histone modification, enriched DNA is amplified, fluorescently labeled and hybridized to tiling microarrays covering promoters or whole chromosomes. Fluorescence intensity profile reflects occupancy across entire genome, enabling comprehensive identification of cistromes, enhancer landscapes and epigenetic domains in single experiment, unlike simple RNA amplification, ELISA detection or proteomic analysis which address fundamentally different molecular questions.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.