#cellular transport

Membrane Transportation

What is the primary function of Na+/K+ ATPase in maintaining secondary active transport?

Primary role of Na+/K+ ATPase in context of secondary active transport is generation and maintenance of electrochemical sodium gradient that powers numerous symporters and antiporters. By pumping three Na+ outward and two K+ inward per ATP hydrolyzed, it creates chemical gradient low intracellular Na+ and electrical gradient interior negative near minus 70 mV. Combined sodium motive force represents stored free energy. Solute carrier families SLC5 SGLT, SLC6 neurotransmitter transporters, SLC38 amino acid transporters, SLC9 NHE and SLC8 NCX exploit this force allowing Na+ to move downhill whil

Ref: Alberts et al., 7th ed., Chapter 11, Na+/K+ pump and secondary transport coupling.

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Membrane Transportation

Which of the following describes V-class ATPases?

V-class ATPases, vacuolar type H+-ATPases, are multi-protein rotary pumps distinct from glucose carriers SGLT or drug exporters ABCB1. They hydrolyze ATP in cytosolic V1 sector comprising A3B3 catalytic hexamer and use released energy to rotate central stalk and translocate protons through membrane-embedded Vo sector containing c-ring proteolipids and subunit a. Cellular localization includes lysosomes, endosomes, trans-Golgi network, synaptic vesicles, secretory granules, osteoclast ruffled border and kidney intercalated cell apical membrane. By acidifying compartments to pH 4.5-5.5 they crea

Ref: Forgac, Nat Rev Mol Cell Biol 2007, V-ATPase function; Nelson & Harvey, Annu Rev Cell Biol 1999.

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Membrane Transportation

What is the function of ABC transporters?

ATP-binding cassette superfamily includes hundreds of members across all domains of life encoded by 49 human genes divided into ABCA to ABCG subfamilies. Core architecture comprises two transmembrane domains forming substrate path and specificity filter, and two cytosolic nucleotide-binding domains containing Walker A P-loop GXXGXGKS/T for phosphate binding, Walker B hhhhDE coordinating Mg2+ and catalytic glutamate, and signature LSGGQ hallmark. ATP binding drives NBD dimerization sandwiching two ATPs, converting TMDs to outward-facing conformation; hydrolysis and release of ADP and Pi resets

Ref: Dean et al., Genome Res 2001, Human ABC family; Rees et al., Nat Rev Mol Cell Biol 2009.

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Membrane Transportation

The ABC transporter superfamily is involved in:

ABC transporter superfamily represents large gene family with 48 members in humans associated with inherited disorders and drug resistance, present also in bacteria where many function as importers. Core architecture comprises two transmembrane domains typically six helices each forming substrate translocation chamber with diverse selectivity filters, and two nucleotide binding domains located cytoplasmically containing Walker A phosphate binding P-loop, Walker B magnesium coordination hhhhhD, signature C motif LSGGQ characteristic of ABC plus H-loop and Q-loop coordinating ATP. Transport cycl

Ref: Higgins, ABC Transporters Annual Review Cell Biol, Lipophilic Drug Export and MDR Phenotype.

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Membrane Transportation

Which of the following ions is not actively transported in most cells?

Cellular ion homeostasis requires continuous energy expenditure to maintain steep gradients. Sodium gradient high outside 145 millimolar low inside 12 millimolar and potassium opposite 4 outside 140 inside established by Na+/K+ ATPase hydrolyzing ATP forming phosphoenzyme E1P E2P cycling three sodium out two potassium in per ATP. Calcium gradient extreme low cytosolic 100 nanomolar versus 1 to 2 millimolar extracellular maintained by plasma membrane Ca2+ ATPase PMCA and sarcoplasmic endoplasmic reticulum Ca2+ ATPase SERCA, both P-type pumps transporting two calcium per ATP against ten thousand

Ref: Alberts et al., Molecular Biology of the Cell, Chapter 11: Ion Distributions - Cl- Passive vs Active Transport.

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Cell Organelles

Late endosomes fuse with:

Endosomal maturation transforms early sorting compartments into degradative organelles preparing cargo for destruction and nutrient recovery. Late endosomes, also termed multivesicular bodies, marked by switch from Rab5 to Rab7 GTPase, more acidic pH near 5.0 to 5.5 due to increased V-ATPase density, and accumulation of intraluminal vesicles formed by ESCRT machinery, move toward microtubule organizing center along microtubules via RILP and dynein. They progressively acquire lysosomal membrane proteins LAMP1, LAMP2 and soluble hydrolases via transport from trans-Golgi network through mannose-6

Ref: Huotari & Helenius, EMBO Journal, 2011: Endosome Maturation and Fusion with Lysosomes.

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Cell Organelles

The voltage-dependent anion channel (VDAC) is located in the:

Exchange of metabolites between cytosol and mitochondria requires regulated permeability across the outer membrane, which unlike inner membrane does not maintain large electrochemical gradient. Voltage-dependent anion channel, VDAC, also called mitochondrial porin, forms the major outer membrane channel present in thousands of copies. Mammalian cells express three isoforms VDAC1, VDAC2 and VDAC3 that assemble as 19-stranded beta-barrel pores with N-terminal helix gating, permitting diffusion of ions, ATP, ADP, succinate, citrate, malate, NADH and other molecules up to about 5 kDa with cut-off

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 14: VDAC and Mitochondrial Metabolite Transport.

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Cytoskeleton

What would be the expected effect of a mutation that prevents dynein’s interaction with dynactin?

Cytoplasmic dynein requires adaptor complexes to achieve efficient cargo transport because motor alone exhibits low processivity and weak cargo binding. Dynactin is twenty three subunit complex containing short Arp1 filament, beta-spectrin adaptor, p150Glued subunit with CAP-Gly microtubule-binding domain and coiled-coil dimerization region that binds dynein intermediate chain. Interaction via extended CC1 fragment of p150Glued locks dynein-dynactin together, increasing run length from submicron to several microns by coordinating two motor domains and suppressing detachment. Preventing this in

Ref: Schroer Annu Rev Cell Dev Biol; dynactin p150Glued enhances dynein processivity and cargo binding.

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Cytoskeleton

The power stroke of dynein is initiated by:

Unlike kinesin or myosin where ATP binding induces neck linker docking lever swing generating force dynein power stroke triggered specifically by ATP hydrolysis chemistry within AAA1 site coupling ring closure to linker movement distinct mechanism. Cycle stages: apo or ADP state linker straight spanning ring from tail to opposite side AAA4-5 high affinity microtubule binding strong attachment. ATP binding closes ring but microtubule already detached due to previous allosteric signaling via stalk. Hydrolysis of ATP to ADP Pi inside AAA1 produces large conformational change transmitted via AAA r

Ref: Carter et al., Science 2011 – Dynein power stroke initiated by hydrolysis of ATP in AAA1 domain mechanism.

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Cytoskeleton

What is the primary function of dynein in cellular transport?

Cytoplasmic dynein 1 is sole minus end directed microtubule motor powering retrograde intracellular transport complementary to kinesin superfamily anterograde transport systems establishing bidirectional highways. Structural features include two identical 530 kDa heavy chains dimerized at tail each with six concatenated AAA ATPase domains forming asymmetric ring linker that swings across ring coiled coil stalk ending in microtubule binding domain MTBD and cargo binding tail associated with intermediate light intermediate and light chains LC8 Tctex. Motor moves toward microtubule minus ends anc

Ref: Roberts et al., Nat Rev Mol Cell Biol 2011 – Cytoplasmic dynein minus end directed transport primary function.

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Cytoskeleton

Which microtubule-binding protein suppresses catastrophe and promotes stability?

Stable microtubule subsets resist cold and nocodazole depolymerization due to decoration by classical structural MAPs reducing dynamics. Members include MAP2 and tau families as well as MAP1B and CLASP. MAP2 specifically expressed in neurons predominantly dendritic compartment forms projection domain protruding from filament serving as spacing crosslinker and microtubule binding repeats with positive charge neutralizing acidic tubulin C terminal tails bridging adjacent protofilaments reinforcing lateral contacts mechanically stiffening filament suppressing catastrophe by maintaining straight l

Ref: Dehmelt & Halpain, Genome Biology 2005 – MAP2 suppresses catastrophe promoting stability via lattice binding.

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Cytoskeleton

What is the primary function of dynein in cellular transport?

Cellular cargo movement includes vesicular trafficking but cilia and flagella beating demands distinct high power motors specialized for sliding instead of cargo carrying. Axonemal dynein evolved from cytoplasmic dynein forms large arrays of outer and inner dynein arms repeating every 96 nm along A tubule of axonemal doublet in nine fold symmetric axoneme. Each arm hydrolyzes ATP in AAA1 to AAA4 rings transmitting conformational change through stalk causing sliding of adjacent B tubule toward base at velocities up to 5 microns per second. Because axoneme constrained by radial spokes linking do

Ref: Ishikawa, Curr Biol – Dynein generates force for cilia flagella movement via axonemal arms.

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