Dedifferentiation in plant tissue culture means:
Dedifferentiation represents reversal of differentiated cell to meristematic, less specialized state capable of renewed division and subsequent redifferentiation into alternative cell types. In vivo, mature specialized cells such as leaf mesophyll or phloem parenchyma that have exited cell cycle and acquired secondary walls, vacuoles, and specific metabolite profiles are induced by wounding or growth regulators to lose specialization traits: they reduce vacuolation, enlarge nucleus, regain dense cytoplasm, and reactivate cell cycle genes cyclin-dependent kinases and cyclins D. In tissue cultur
Ref: Taiz & Zeiger Physiology – Dedifferentiation; Sugimoto et al. 2010 Plant Cell dedifferentiation mechanism
