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#callus

2 public questions tagged with this topic.

Plant tissue culture 3

Friable callus is mainly used to initiate:

Texture of callus determines suitability for liquid systems. Compact nodular callus consists of tightly packed cells cemented by calcium pectate, resistant to dispersal. Friable type arises when pectin methylesterase activity reduces middle lamella cohesion, producing crumbly masses of loosely associated, highly vacuolated cells with large intercellular air spaces. Upon transfer to agitated liquid Murashige-Skoog medium containing 2,4-D, these fragments break easily into single cells and small aggregates under 100 micrometer diameter, forming homogeneous suspension. Such suspensions exhibit ex

Ref: Murashige & Skoog 1962 friable callus; Street Plant Tissue 1977 suspension initiation; Dodds & Roberts Experiments 4th ed; NCBI NBK26844 cell suspensions texture importance.

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Plant tissue culture 1

Callus is best defined as:

Callus is unorganized proliferation of dedifferentiated parenchymatous cells produced in response to wounding or exogenous hormone application in vitro under sterile conditions. When explant such as leaf segment, stem internode, or cotyledon is placed on Murashige and Skoog medium supplemented with balanced auxin like 2,4-D and cytokinin BAP, mature cells dedifferentiate and divide mitotically forming amorphous mass of thin-walled, vacuolated, isodiametric cells lacking organized meristems, vascular pattern, or polarity initially. Callus growth reflects loss of positional information and conti

Ref: Bhojwani SS & Razdan MK. Plant Tissue Culture – callus definition. George EF et al. Plant Propagation – unorganized mass

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