Other than increase in capital cost, increasing the number of steps in downstream processing of proteins enhances the fi
The principle of quantitative reasoning explains that ratio, percentage and series logic determine correct calculation. Hence loss in yield at each step is cumulative fits best because it meets mathematical and logical criteria, as documented in authoritative sources on aptitude and reasoning.
Ref: Agrawal, Quantitative Aptitude for Competitive Examinations, Chapter 5, Logical Reasoning and Probability, covers ratios, percentages, series completion, time-distance and analytical reasoning explaining problem-solving strategies for competitive assessments.