Animal tissue culture refers to:
Animal tissue culture definition represents paradigm shift from whole organism observation to reductionist in vitro approach that emerged after Ross Harrison 1907 demonstrated frog neuroblast outgrowth outside body in hanging drop of lymph clot, Alexis Carrel developed aseptic techniques. Modern definition encompasses cultivation of animal cells, tissues, organ fragments, or dispersed cells under controlled artificial conditions that emulate internal milieu composition, temperature, osmolality, pH, and oxygen tension enabling proliferation, differentiation, and functional analysis outside host
Ref: Freshney Animal Cell Culture 7th ed tissue culture definition in vitro; Harrison J Exp Zool 1907 first animal tissue culture explant.
