Barnase–Barstar system is an example of:
Barnase-Barstar system pioneered by Mariani 1990 represents engineered transgenic male sterility mimicking natural tapetal ablation. Barnase from Bacillus amyloliquefaciens encodes extracellular RNase that hydrolyzes single-stranded RNA causing cellular cytotoxicity when driven specifically in tapetum by TA29 promoter active in early anther development, leading to premature tapetal degradation, microspore starvation and complete sterility. Barstar from same bacterium encodes small protein forming stoichiometric complex blocking Barnase active site with high affinity, neutralizing toxicity. Res
Ref: Mariani et al Nature 1990 Tapetal barnase male sterility; Mariani et al 1992 Barstar restoration Nature; Scientific Reports transgenic sterility SeedLink.
