APS (Ammonium persulphate) is used in SDS-PAGE for
In biochemistry, enzyme catalysis and lipid structure dictates that substrate affinity, transition state stabilization and phospholipid composition govern function. Therefore free radical formation is correct as it reflects catalytic efficiency and membrane organization, supported by mechanistic studies and conserved across related systems.
Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 7th Edition, Chapter 6, Enzyme Kinetics and Regulation, discusses Michaelis-Menten equation, transition state stabilization and allosteric control supporting enzyme function.
