Which protein is responsible for the intracellular movement of Listeria by actin polymerization?
Intracellular pathogenesis of Listeria monocytogenes illustrates actin based propulsion mechanism. After internalization via E cadherin or Met receptor mediated endocytosis, bacterium escapes vacuole via pore forming toxin listeriolysin O and phospholipases PlcA PlcB, enters cytosol and replicates. It then expresses ActA protein anchored via C terminal transmembrane domain, retained at old pole after secretion. ActA N terminal domain mimics eukaryotic nucleation promoting factor recruiting and activating host Arp2/3 complex via acidic motifs and binding Ena VASP via proline rich repeats accele
Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 16: Listeria ActA and Actin-Based Motility.
