Skip to content
Login Register

#abzymes

2 public questions tagged with this topic.

Immunological Applications

Abzymes are antibodies that:

Catalytic antibodies termed abzymes represent attempt to harness immune binding energy for chemical transformation similar to enzymes that accelerate reactions 10^10 fold by stabilizing transition state through complementarity. Natural enzymes use preorganized active site residues histidine, aspartate, serine triad precisely positioned via folding to provide general acid-base, nucleophile, oxyanion hole stabilization. Abzymes generated by immunization with stable transition-state analogue designed to mimic high energy tetrahedral intermediate of ester hydrolysis such as phosphonate monoester w

Ref: Lerner et al Science 1991 252:659 catalytic antibodies abzyme; Shabat et al Transition state analog phosphonate ester.

View Details
Immunological Applications

Abzymes are antibodies that:

Abzymes catalytic antibodies merge Ig antigen-binding specificity enzyme-like rate acceleration accelerating chemical transformations difficult natural enzymes. Generation exploits transition state analog theory Pauling: enzyme binds transition state tighter substrate lowering activation energy. Immunization stable mimic tetrahedral high-energy intermediate ester hydrolysis phosphonate ester phosphorus replacing carbon maintaining tetrahedral but stable replaces labile carbonyl elicits immune response producing antibodies binding pocket complementary transition state geometry providing binding

Ref: Tramontano Science 1986 Abzyme Catalytic Antibody First Demonstration; Lerner Chem Review Abzymes Concept 1992; Janeway Abzyme Catalytic Activity Binding Energy Chapter 5.

View Details