Practice question
Question
The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is _________ Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln
Explanation
Trypsin cuts after Lys and Arg, producing three peptides. CNBr cleaves after Met within first two peptides, splitting each into two, yielding total five fragments. Sequential cleavage demonstrates how protease specificity determines fragment count for protein mapping and sequencing strategies.
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