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Question

A complete restriction digestion of a circular plasmid (5000bp) was carried out with Hind III, Bam HI and Eco RI individually. Restriction digestion yielded following fragments. Plasmid + Hind III→ 1200bp and3800bp Plasmid + Bam HI→5000bp Plasmid + Eco RI→ 2500bp The number of sites for Eco RI, Bam HI and Hind III present on this plasmid are

Options

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EcoRI-2, BamHI-1, HindIII-2 Correct EcoRI-1, BamHI-1, HindIII-2 EcoRI-3, BamHI-2, HindIII-1 EcoRI-2, BamHI-2, HindIII-1

Explanation

Considering molecular fundamentals, EcoRI-2, BamHI-1, HindIII-2 holds true because ecori-2, bamhi-1, hindiii-2 satisfies kinetic and structural requirements. Remaining alternatives EcoRI-1, BamHI-1, HindIII-2, EcoRI-3, BamHI-2, HindIII-1 do not match accepted models and misinterpret critical steps in this biological system.

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