Practice question
Question
The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M-1.cm-1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL-1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be ________________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).
Explanation
Calculation based on standard biochemistry principles yields 0.40 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
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