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Practice question

Question

Decimal reduction time of bacterial spores is 23 min at 121 ⁰C and the death kinetics follow first order. One liter medium containing 10^5 spores per mL was sterilized for 10 min at 121 ⁰C in a batch sterilizer. The number of spores in the medium after sterilization (assuming destruction of spores in heating and cooling period is negligible) will be ___________×10^7.

Explanation

Calculation based on standard microbiology principles yields 3.67 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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