Practice question

TATA-binding protein functions within multisubunit TFIID complex comprising TBP plus thirteen to fourteen TBP-associated factors TAF1 to TAF14 forming lobe-like structures visible by electron microscopy. TFIID is first general transcription factor to bind core promoter, recognizing TATA box via TBP, initiator via TAF1 and TAF2, downstream promoter element via TAF6 and TAF9. TFIIA stabilizes TFIID-DNA interaction while TFIIB joins. Formation of TBP-TFIID promoter complex is rate-limiting step regulated by activators, coactivators like Mediator, and chromatin remodeling events controlling polymerase recruitment.