Practice question
Question
Match List I with List II: List I (Chromatography) List II (Buffer system) A. Ion Exchange chromatography I. Isocratic buffer B. Gel permeation chromatography II. Increasing salt gradient C. Chromatofocussing III. Reducing polarity of elution buffer D. Hydrophobic interaction chromatography IV. Buffer containing ampholytes Choose the correct answer from the options given below
Explanation
enzyme catalysis and lipid structure analysis shows substrate affinity, transition state stabilization and phospholipid composition govern function. Consequently A-II, B-I, C-IV, D-III emerges as the valid choice since it reflects catalytic efficiency and membrane organization, aligning with established principles in biochemistry literature.
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