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MCQ

Question

In the laboratory setting, you are provided with isolated intact mitochondria, and oxidizable substrates such as pyruvate or succinate. Addition of 02 and an oxidizable substrate to the isolated intact mitochondria results in a net synthesis of ATP. What can be normally expected, if a small amount of detergent is added to make the membranes leaky ?

Options

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Electron transport will stop, but oxidation of metabolites will still take place. ATP production will be reduced. The transmembrane proton concentration gradient will be lost, but membrane electric potential will be maintained. ATP production will be reduced. Both electron transport and oxidation of metabolites by oxygen will stop. No ATP production takes place. Both electron transport and oxidation of metabolites by oxygen will continue. However, no ATP will be made. Correct

Explanation

enzyme catalysis and lipid structure analysis shows substrate affinity, transition state stabilization and phospholipid composition govern function. Consequently Both electron transport and oxidation of metabolites by oxygen will continue.... emerges as the valid choice since it reflects catalytic efficiency and membrane organization, aligning with established principles in biochemistry literature.

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