Practice question

MCQ

Question

In DNA foot-printing, A The DNA is labelled by random priming so that the entire DNA is labelled and one does not miss out any region that binds with the given protein. B. The DNA is end-labelled so that the bands get organized from higher to lower size after electrophoresis and autoradiography. C. A sequencing polyacrylamide gel is used to resolve all the fragments distinctly. D. A higher concentra1ion of agarose gel is used to resolve the finer bands. Which one of the following options has the combination of all correct statements?

Options

Choose one · Correct answer highlighted

A only B and D B and C Correct D only

Explanation

in dna foot-printing, a the dna is operates via end-labeled probe generates nested fragments on sequencing PAGE revealing protected gaps. DNase I protection validates option C. Other options propose random priming, inconsistent with published kinetics and mutant phenotypes.

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