Practice question
Question
In an attempt to detect protein expression profile in a cell, Western blot technique is employed. Expression of two new proteins is to be followed by probing with respective high affinity antibodies (raised in rabbit). Unfortunately, the two proteins were found to co-migrate in SDS-PAGE profile. Under this situation, using one dimensional SDSPAGE and by Western blot, which one of the following is the best way to demonstrate the presence of both the proteins?
Explanation
in an attempt to detect protein expression operates via Subjecting the technique of stripping/re-probbing of the gel represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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