Practice question
Question
During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of genes and their tissue-specific transcription. The following are the possible methods used for the determination. A. Polymerase Chain Reaction(PCR) B. Southern blot hybridization C. Reverse Transcriptase PCR D. Western blot Choose the correct set of combinations.
Explanation
during transgenesis, the location of the genes operates via B and C represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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