Practice question
Question
During apoptosis, phosphatidyl serine (PS) usually present in the inner leaflet of the plasma membrane flips to the outer membrane. Annexin V is a protein that binds to PS, Using this as a tool, we identifiy the apoptic cells from necrotic and normal cell populations by FACS using FITC-tagged Annexin V. Propidium iodide (PI) is used to stain the nucleus which generally identifies necrotic and late apoptic cells. In which area of the plot you should get early apoptic cells by FACS analysis?
Explanation
during apoptosis, phosphatidyl serine (ps) usually present operates via Quadrant III represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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