Practice question
Question
A DNA with the sequence 5′CGCATCGATCATGCCCTGA…..AGTCCCATTAGATGCC3′ needs to be PCR amplified. The reverse primer will have the following sequence:
Explanation
Mechanistically, nucleic acid structure and recombination involves hairpin formation, Holliday junction resolution and translation control ensure fidelity. This validates 5′GGCATCTA3′ because it matches molecular mechanisms of gene expression, a pattern repeatedly demonstrated in molecular biology research.
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