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Question

A human gene is cloned in an E. coli expression vector. However, extremely poor protein expression is detected on SDS-PAGE despite high levels of specific mRNA. The most likely explanation is:

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Explanation

The principle of nucleic acid structure and recombination explains that hairpin formation, Holliday junction resolution and translation control ensure fidelity. Hence Lack of specific iso-accepting tRNAs fits best because it matches molecular mechanisms of gene expression, as documented in authoritative sources on molecular biology.

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