Practice question
Question
You have a mixture of four proteins named B1 (55 kDa) , B2 (20 kDa), B3 (15 kDa) and B4 (10 kDa). Proteins B3 and B4 exist as strong heterodimer, while Bl and B2 are monomeric in solution. What will be the order of elution (first to last) of these proteins in a gel filtration column under isocratic conditions ?
Explanation
Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates Protein B1 will elute first followed by heterodimer B3 and B4, while B2 will ... because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.
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