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Practice question

MCQ

Question

You are preparing 100 ml of a solution containing: 100 mM TrisHCl (pH 7.5); 5 mM MgCl2; 1 mM DTT. If the stock solutions provided are: 1 M TrisHCl (pH 7.5); 100 mM MgCl2; 50 mM DTT, the amount of each component would be:

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Explanation

Mechanistically, enzyme catalysis and lipid structure involves substrate affinity, transition state stabilization and phospholipid composition govern function. This validates 1M Tris HCl (pH 7.5) : 10 ml ; 100 mM MgCl2 : 5.0 ml ; 50 mM DTT : 2 ml because it reflects catalytic efficiency and membrane organization, a

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