Practice question

MCQ

Question

Which among the following statements are correct about very long DNA fragments (>100 kb) from eukaryotic genomes? (A) They can be efficiently packaged in a λ phage vector. (B) They can be propagated in a yeast artificial chromosome. (C) They must have cohesive ends for cloning. (D) They can be separated by pulsed field gel electrophoresis technique. Choose the correct answer from the options given below :

Options

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(A) and (B) only (A) and (D) only (C) and (D) only (B) and (D) only Correct

Explanation

Fragments exceeding 100 kb cannot be packaged efficiently in lambda phage vectors limited to about 45 kb. Yeast artificial chromosomes accommodate megabase inserts with centromere and telomeres. Such large fragments are resolved by pulsed field gel electrophoresis which alternates electric field direction.

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