Practice question

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Question

The specific activity of an enzyme in a crude extract of E. co/i is 9.5 units/mg of protein. The specific activity increased to 68 units/mg of protein upon ion-exchange chromatography. The fold purification is

Explanation

Calculation based on standard biochemistry principles yields 7.16 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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