Practice question
Question
The most important property of any microscope is its resolution (D) and can be calculated from the formulaD= 0.61λ/ N sinαwhere D is, minimum distance between two distinguishable objects,A. is the wave length of incident light, a is, the angular aperture and N is the refractive index of the medium. Given below are several suggestions to improve the resolution of a microscope: A. decrease the wave length of incident light B. increase the wave length of incident light C. use oil which has a higher refractive index D. use oil because of its lower refractive index Which one is the correct suggestion?
Explanation
In the most important property of any microscope, A and C represents established outcome. functional assays confirms option A (A and C) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
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